Antibody Saturation — EC90 / 4PL Curve Fitter

Fit your Simple Western antibody titration data with EC90 and 4PL models, get recommended dilutions, and optionally contribute to a shared antibody database.

How It Works

Watch the overview video to understand antibody saturation and the EC90 concept.

1 Python Environment

Runs Python (NumPy, SciPy, Matplotlib) directly in your browser — no data leaves your computer.

First visit? The Python environment (~30 MB) downloads once and is cached. Takes ~20–30 s on first load.
Loading Python environment…

2 Paste Peak Data

Copy peak data from Compass and paste below.

  1. In Compass, open the Peaks table for your titration experiment.
  2. Ensure columns: Sample, Name, Primary Attr (or Primary), Area, Height, Baseline, S/N.
  3. Select all rows + header, Copy (Ctrl+C).
  4. Paste below and click Run Analysis.

Dilution values must be in 1:100 or 1/100 format.

3 Results

Curve fits, recommended dilutions, and baseline analysis.

📊 Contribute to the antibody database?

Your graphs stay on your computer — nothing is shared without your consent.
Optionally send anonymised results (graphs + antibody info) to help build a shared reference database. No sample identity or patient data is collected.

Console Output